ACADSTAFF UGM
| Title | : | Activities of Plant Cell Wall Degrading Enzymes by Bacterial Soft-rot of Orchid |
| Author | : |
Prof. Dr. Tri Joko, S.P., M.Sc. (1) AHMAD SUBANDI (2) RR NANDA KUSUMANDARI (3) Dr. Ir. Arif Wibowo, M.Agr.Sc. (4) Prof. Ir. Achmadi Priyatmojo, M.Sc., Ph.D. (5) |
| Date | : | 0 2014 |
| Keyword | : | soft rot,orchid,plant cell wall-degrading enzymes,16S rDNA soft rot,orchid,plant cell wall-degrading enzymes,16S rDNA |
| Abstract | : | Soft rot by bacterial pathogens is one of the most widespread and destructive diseases on various plants including orchids throughout the world. The pathogenicity of the pathogens is reported to be mainly determined by massive production of plant cell wall-degrading enzymes (PCDE). In the previous work, we have isolated 20 isolates of bacterial soft rot from orchids collected in Yogyakarta Special Region and West Java province, Indonesia. In this study, we further confirmed them as pathogens by hypersensitive reaction assay on tobacco leaves followed by pathogenicity test on Phalaenopsis sp. The production of four major PCDE by qualitative plate assays including pectate lyase, polygalacturonase, cellulase and protease was also evaluated. Even though all the isolates were able to initiate soft rot symptom, our results showed two distinct groups which clustered as producing and non-producing PCDE. The 16S rDNA analysis revealed that the isolates belonged to the genera Pectobacterium, Klebsiella, Serratia, Enterobacter, Citrobacter, Providencia and Pseudomonas. |
| Group of Knowledge | : | Ilmu Hama dan Penyakit Tanaman |
| Original Language | : | English |
| Level | : | Internasional |
| Status | : |
Published
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| 1 |
TriJoko-print edition.pdf
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1 Activities of plant cell wall-degrading enzymes by bacterial soft rot of orchid.pdf
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Activities of plant cell wall-degrading enzymes by bacterial soft rot of orchid.pdf
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| 4 |
20141001_Editorial Board Archives of Phytopathology and Plant Protection Volume 47 nomor 10.pdf
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20141001_Table of Content Archives of Phytopathology and Plant Protection Volume 47 nomor 10.pdf
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Activities of plant cell wall-degrading enzymes by bacterial soft rot of orchid.pdf
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