ACADSTAFF UGM

CREATION
Title : An Attempt to Establish the Lipase Gene Sequence of Alcaligenes Sp. JG3 Using Internal Primer
Author :

NORMAN YOSHI HARYONO (1) Dr. Winarto Haryadi, M.Si. (2) Prof. Tri Joko Raharjo, S.Si., M.Si., Ph.D. (3)

Date : 0 2019
Keyword : Alcaligenes Sp. JG3, Internal Primer, Lipase, PCR Alcaligenes Sp. JG3, Internal Primer, Lipase, PCR
Abstract : Alcaligenes sp. JG3 is a local strain bacterium from Indonesia, isolated from cultivated corn field of Central Java. This bacterium is able to produce lipase with fairly high activity. In order to do lipase gene sequence characterization, two sets of primer pair were used in this study (primer Fjg3 5’- ATGACCGAGCTGACTGTAG-3’, Rjg3 5’-TCAGGAGGGGTAAATCCAC-3’ and internal primer Fi 5’-TGACCCATGACCAGGCGGAA-3’ and Ri 5’-TTCGCCTGGTCATGGGTCA-3’). The complete lipase JG3 gene sequence consists of 1081 bp from start codon ATG to the stop codon of TGA. Lipase JG3 had high similarity to another lipase from genus Alcaligenaceae which was up to 90%. However, the 3D protein visualization analysis indicated that this lipase JG3 also has the characteristic of ABC transporter protein.
Group of Knowledge : Kimia
Original Language : English
Level : Internasional
Status :
Published
Document
No Title Document Type Action
1 Tri Joko Material Science Forum 948 57-62.pdf
Document Type : [PAK] Full Dokumen
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2 similarity_An Attempt to Establish the Lipase Gene Sequence of Alcaligenes Sp_ JG3 Using Internal Primer.pdf
Document Type : [PAK] Cek Similarity
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