ACADSTAFF UGM
Severity: Warning
Message: fopen(/var/cpanel/php/sessions/ea-php73/PHPSESSID6c4390e018a0d9391e5ba849f731a056): failed to open stream: No space left on device
Filename: drivers/Session_files_driver.php
Line Number: 157
Backtrace:
File: /home1/ditlitluaracadst/public_html/acadstaff/application/third_party/MX/Loader.php
Line: 173
Function: _ci_load_library
File: /home1/ditlitluaracadst/public_html/acadstaff/application/third_party/MX/Loader.php
Line: 190
Function: library
File: /home1/ditlitluaracadst/public_html/acadstaff/application/third_party/MX/Loader.php
Line: 153
Function: libraries
File: /home1/ditlitluaracadst/public_html/acadstaff/application/third_party/MX/Loader.php
Line: 65
Function: initialize
File: /home1/ditlitluaracadst/public_html/acadstaff/application/third_party/MX/Base.php
Line: 55
Function: __construct
File: /home1/ditlitluaracadst/public_html/acadstaff/application/third_party/MX/Base.php
Line: 60
Function: __construct
File: /home1/ditlitluaracadst/public_html/acadstaff/application/third_party/MX/Controller.php
Line: 4
Function: require
File: /home1/ditlitluaracadst/public_html/acadstaff/application/third_party/MX/Modules.php
Line: 134
Function: include_once
File: /home1/ditlitluaracadst/public_html/acadstaff/application/core/MY_Controller.php
Line: 5
Function: spl_autoload_call
File: /home1/ditlitluaracadst/public_html/acadstaff/index.php
Line: 318
Function: require_once
Severity: Warning
Message: session_start(): Failed to read session data: user (path: /var/cpanel/php/sessions/ea-php73)
Filename: Session/Session.php
Line Number: 141
Backtrace:
File: /home1/ditlitluaracadst/public_html/acadstaff/application/third_party/MX/Loader.php
Line: 173
Function: _ci_load_library
File: /home1/ditlitluaracadst/public_html/acadstaff/application/third_party/MX/Loader.php
Line: 190
Function: library
File: /home1/ditlitluaracadst/public_html/acadstaff/application/third_party/MX/Loader.php
Line: 153
Function: libraries
File: /home1/ditlitluaracadst/public_html/acadstaff/application/third_party/MX/Loader.php
Line: 65
Function: initialize
File: /home1/ditlitluaracadst/public_html/acadstaff/application/third_party/MX/Base.php
Line: 55
Function: __construct
File: /home1/ditlitluaracadst/public_html/acadstaff/application/third_party/MX/Base.php
Line: 60
Function: __construct
File: /home1/ditlitluaracadst/public_html/acadstaff/application/third_party/MX/Controller.php
Line: 4
Function: require
File: /home1/ditlitluaracadst/public_html/acadstaff/application/third_party/MX/Modules.php
Line: 134
Function: include_once
File: /home1/ditlitluaracadst/public_html/acadstaff/application/core/MY_Controller.php
Line: 5
Function: spl_autoload_call
File: /home1/ditlitluaracadst/public_html/acadstaff/index.php
Line: 318
Function: require_once
| Title | : | An Attempt to Establish the Lipase Gene Sequence of Alcaligenes Sp. JG3 Using Internal Primer |
| Author | : |
NORMAN YOSHI HARYONO (1) Prof. Dr. Drs. Winarto Haryadi, M.Si. (2) Prof. Tri Joko Raharjo, S.Si., M.Si., Ph.D. (3) |
| Date | : | 0 2019 |
| Keyword | : | Alcaligenes Sp. JG3, Internal Primer, Lipase, PCR Alcaligenes Sp. JG3, Internal Primer, Lipase, PCR |
| Abstract | : | Alcaligenes sp. JG3 is a local strain bacterium from Indonesia, isolated from cultivated corn field of Central Java. This bacterium is able to produce lipase with fairly high activity. In order to do lipase gene sequence characterization, two sets of primer pair were used in this study (primer Fjg3 5’- ATGACCGAGCTGACTGTAG-3’, Rjg3 5’-TCAGGAGGGGTAAATCCAC-3’ and internal primer Fi 5’-TGACCCATGACCAGGCGGAA-3’ and Ri 5’-TTCGCCTGGTCATGGGTCA-3’). The complete lipase JG3 gene sequence consists of 1081 bp from start codon ATG to the stop codon of TGA. Lipase JG3 had high similarity to another lipase from genus Alcaligenaceae which was up to 90%. However, the 3D protein visualization analysis indicated that this lipase JG3 also has the characteristic of ABC transporter protein. |
| Group of Knowledge | : | Kimia |
| Original Language | : | English |
| Level | : | Internasional |
| Status | : |
Published
|