Title | : | ANTIBACTERIAL PEPTIDE FROM SOLID PHASE EXTRACTION (SPE) FRACTIONATION ON TRYPSIN HYDROLYSIS OF JATROPHA (Ricinus communis) SEED PROTEIN ACID EXTRACT |
Author | : |
DENNA RAY ATMAWATI (1) ZEHAN ANDRIANA (2) Respati Tri Swasono, S.Si., M.Phil., Ph.D (3) Prof. Tri Joko Raharjo, S.Si., M.Si., Ph.D. (4) |
Date | : | 0 2022 |
Keyword | : | TFA Extraction, Anti-Bacterial Peptide, Ricinus Communis, Cationic Exchange, SPE TFA Extraction, Anti-Bacterial Peptide, Ricinus Communis, Cationic Exchange, SPE |
Abstract | : | The antimicrobial peptide is a new potential antibiotic type that can be isolated not only from free peptides but also peptides from hydrolyzed protein with proteolytic enzymes. The protein of jatropha seed is toxic due to RIP, ribosomeinactivating protein, and a potential source of AMP. Trifluoroacetic acid was used to isolate jatropha seed protein. The protein was hydrolyzed using trypsin and the hydrolysate separated with a cation exchange solid phase extraction (SPE) column. Each fraction was tested for antimicrobial activity and resulted in the pH 3 and pH 4 fractions being able to inhibit bacterial growth against Staphylococcus aureus (Gram-positive bacteria) and Escherichia coli (Gramnegative bacteria). The pH 3 fraction has IC50 value 17.95 µg/mL to E. coli and 17.03 µg/mL to S. aureus, while the pH fraction has IC50 value 37.30 µg/mL and 95.31 µg/mL respectively. There are three peptides amino acid sequences GAGLVPKR, MGACCSKEPSFAEGR and VALASLLSQPLPQISDK identified from those fractions. These peptides potentially inhibit bacterial growth through membrane destabilization mechanism, due to their amphipathic and ionic properties |
Group of Knowledge | : | Kimia |
Original Language | : | English |
Level | : | Internasional |
Status | : |
Published
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No | Title | Action |
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1 |
2_ RJC Editorial Board_opt.pdf
Document Type : [PAK] Halaman Editorial
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2 |
1_ Manuskrip RJC 2022 15 (2) 1288.pdf
Document Type : [PAK] Full Dokumen
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3 |
3_ RJC TOC 2022 15 (2)_opt.pdf
Document Type : [PAK] Daftar Isi
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4 |
6_ Tunitin RJC 2022 15 (2) 1288.pdf
Document Type : [PAK] Cek Similarity
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5 |
8_ BUKTI KORESPONDENSI RJC 2022 15 (2) 1288_compressed.pdf
Document Type : [PAK] Bukti Korespondensi Penulis
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6 |
form-L1-permohonan_penghargaan-karya-ilmiah-sudah-terbit-2022 TJR RJC.pdf
Document Type : Dokumen Pendukung Karya Ilmiah (Hibah, Publikasi, Penelitian, Pengabdian)
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7 |
PEER REVIEW_tri joko tambahan 1.pdf
Document Type : [PAK] Peer Review
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