ACADSTAFF UGM

CREATION
Title : ANTIBACTERIAL PEPTIDE FROM SOLID PHASE EXTRACTION (SPE) FRACTIONATION ON TRYPSIN HYDROLYSIS OF JATROPHA (Ricinus communis) SEED PROTEIN ACID EXTRACT
Author :

DENNA RAY ATMAWATI (1) ZEHAN ANDRIANA (2) Respati Tri Swasono, S.Si., M.Phil., Ph.D (3) Prof. Tri Joko Raharjo, S.Si., M.Si., Ph.D. (4)

Date : 0 2022
Keyword : TFA Extraction, Anti-Bacterial Peptide, Ricinus Communis, Cationic Exchange, SPE TFA Extraction, Anti-Bacterial Peptide, Ricinus Communis, Cationic Exchange, SPE
Abstract : The antimicrobial peptide is a new potential antibiotic type that can be isolated not only from free peptides but also peptides from hydrolyzed protein with proteolytic enzymes. The protein of jatropha seed is toxic due to RIP, ribosomeinactivating protein, and a potential source of AMP. Trifluoroacetic acid was used to isolate jatropha seed protein. The protein was hydrolyzed using trypsin and the hydrolysate separated with a cation exchange solid phase extraction (SPE) column. Each fraction was tested for antimicrobial activity and resulted in the pH 3 and pH 4 fractions being able to inhibit bacterial growth against Staphylococcus aureus (Gram-positive bacteria) and Escherichia coli (Gramnegative bacteria). The pH 3 fraction has IC50 value 17.95 µg/mL to E. coli and 17.03 µg/mL to S. aureus, while the pH fraction has IC50 value 37.30 µg/mL and 95.31 µg/mL respectively. There are three peptides amino acid sequences GAGLVPKR, MGACCSKEPSFAEGR and VALASLLSQPLPQISDK identified from those fractions. These peptides potentially inhibit bacterial growth through membrane destabilization mechanism, due to their amphipathic and ionic properties
Group of Knowledge : Kimia
Original Language : English
Level : Internasional
Status :
Published
Document
No Title Document Type Action
1 2_ RJC Editorial Board_opt.pdf
Document Type : [PAK] Halaman Editorial
[PAK] Halaman Editorial View
2 1_ Manuskrip RJC 2022 15 (2) 1288.pdf
Document Type : [PAK] Full Dokumen
[PAK] Full Dokumen View
3 3_ RJC TOC 2022 15 (2)_opt.pdf
Document Type : [PAK] Daftar Isi
[PAK] Daftar Isi View
4 6_ Tunitin RJC 2022 15 (2) 1288.pdf
Document Type : [PAK] Cek Similarity
[PAK] Cek Similarity View
5 8_ BUKTI KORESPONDENSI RJC 2022 15 (2) 1288_compressed.pdf
Document Type : [PAK] Bukti Korespondensi Penulis
[PAK] Bukti Korespondensi Penulis View
6 form-L1-permohonan_penghargaan-karya-ilmiah-sudah-terbit-2022 TJR RJC.pdf
Document Type : Dokumen Pendukung Karya Ilmiah (Hibah, Publikasi, Penelitian, Pengabdian)
Dokumen Pendukung Karya Ilmiah (Hibah, Publikasi, Penelitian, Pengabdian) View
7 PEER REVIEW_tri joko tambahan 1.pdf
Document Type : [PAK] Peer Review
[PAK] Peer Review View