ACADSTAFF UGM
| Title | : | Antibiofilm activity of (1)-N-2-methoxybenzyl-1,10-phenanthrolinium bromide against Candida albicans |
| Author | : |
Prof. dr. Titik Nuryastuti, M.Si., Ph.D, SpMK(K) (1) Setiawati (2) Prof. dr. Ngatidjan, M.Sc.Sp.FK(K) (3) Prof. Dr. Mustofa, Apt., M.Kes. (4) Prof. Drs. Jumina, Ph.D. (5) Dhina Fitriastuti (6) Dr. Muhammad Idham Darussalam Mardjan S.Si., M.Sc. (7) |
| Date | : | 2018 |
| Keyword | : | 1,10-phenanthroline,antibiofilm,candida albicans 1,10-phenanthroline,antibiofilm,candida albicans |
| Abstract | : | The therapy for invasive candidiasis related to biofilms infection remains a difficult medical problem. To overcome this problem, efforts have been made to search for novel antibiofilm agents from various sources. This study investigated the in vitro antibiofilm activity of (1)-N-2-methoxybenzyl-1,10-phenanthrolinium bromide (FEN) against Candida albicans. The minimum biofilm inhibitory concentration (MBIC) and minimum biofilm reduction concentration (MBRC) were determined using the MTT (3-(4-5-dimethylthiazol-2-yl)2,5-dyphenyl tetrazolium bromide) reduction assay. Biofilms on surfaces were visualized using scanning electron microscopy (SEM). This new compound inhibited the growth of C. albicans biofilms by 80 % with an MBIC80 range from 0.5–2.0 ?g/mL. The ability of FEN to reduce 50 % of a preformed biofilm was demonstrated by defining a MBRC50 range from 6.25–-12.5 ?g/mL. To reduce 80 % of a preformed biofilm required higher concentrations > 200 ?g/mL. In addition, SEM images showed disruption of C. albicans biofilms matrix exposed to FEN. These results indicated that (1)-N-2-methoxybenzyl-1,10-phenanthrolinium bromide has the potential to be developed as a new antibiofilm agent against C. albicans. |
| Group of Knowledge | : | Kimia |
| Original Language | : | English |
| Level | : | Internasional |
| Status | : |
Published
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