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Title : Antioxidant Activity and Histochemical Analysis of Acalypha indica L. and Acalypha wilkesiana Muell. Arg. Vegetative and G enerative Organs
Author :

Prof. Dr. Yekti Asih Purwestri, S.Si., M.Si. (1) Prof. Dr. Laurentius Hartanto Nugroho, M.Agr. (2) NOVIA AGUSTINA (3)

Date : 0 2016
Keyword : Acalypha indicaL., Acalypha wilkesianaMuell.Arg, antioxidant, secondary metabolites, histochemical. Acalypha indicaL., Acalypha wilkesianaMuell.Arg, antioxidant, secondary metabolites, histochemical.
Abstract : Acalypha indicaL. and Acalypha wilkesiana Muell.Arg.which are member fEuphorbiaceae have ethnopharmacological history. However, the use of Acalypha indica L. and Acalypha wilkesiana Muell.Arg. have not been ptimalized. The objective of this study was to know the antioxidant activity, econdary metabolites content, the location and distribution of secondary etabolites, and secretory structure of Acalypha indicaL. and Acalypha ilkesianaMuell.Arg. Antioxidant activity were determined by DPPH methode sing chloroform and methanol extracts of stems , leaves, and the mixture of flowers-fruits-seeds. Secondary metabolitescontents were tested using arious reagent and measured using spectrophotometre. Moreover,the istribution of secondary metaboliteswere analyzed by histochemical test.DPPH est showed that methanolic extract of leaves and the mixture of flowers-fruits-seeds of Acalypha wilkesiana performed the bestantioxidant activitycompared to other samples. The IC50value of leaves and flowers-fruits-seeds mixture methanolic extracts were 3,42±0,78 ?g/ml and4,71±0,71 ?g/ml respectively. The phenol content of leaves and the mixture of flowers -fruits-seeds methanolic extract of Acalypha wilkesianawere 238,89±19,48%GAE nd301,22±32,16%GAErespectively; flavonoids content were 36,93±2,01%QEand 58,67±1,51%QErespectively; and tannin content were 84,18±5,68%GAE and60,96±0,67%GAErespectively. Antioxidant activity could be due to the phenol, flavonoids, and tannin contents. The histochemical test showed that henol, flavonoids, tannin, and alkaloids could be found in the stems, leaves, and the mixture of flowers-fruits-seeds of Acalypha indica and Acalypha wilkesiana. The distribution of various mentioned group of compound were lmost in the whole of various tissues. The secretory stuctures were consisted finternal structure and external structure which in the forrm of capitate landular trichome.Keywords: Acalypha indicaL., Acalypha wilkesianaMuell.Arg, antioxidant, secondary metabolites, histochemical.
Group of Knowledge : Biologi (dan Bioteknologi Umum)
Original Language : English
Level : Internasional
Status :
Published
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