ACADSTAFF UGM
| Title | : | Application of silica extracted from rice husk ash for the encapsulation of AFB1 antibody as a matrix in immunoaffinity columns |
| Author | : |
Dr. Deni Pranowo, S.Si., M.Si. (1) Prof. Dr.rer.nat. Nuryono, M.S. (2) Prof. Dr. Ir. Ali Agus, DAA., DEA., IPU., ASEAN Eng. (3) Prof. Drs. Jumina, Ph.D. (4) Romsyah Maryam (5) Dr.nat.techn. Francis M Constance Sigit Setyabudi, S.T.P., M.P. (6) |
| Date | : | 2017 |
| Keyword | : | aflatoxin B1, immunoaffinity column, rice husk ash, silica ge,sol-gel aflatoxin B1, immunoaffinity column, rice husk ash, silica ge,sol-gel |
| Abstract | : | The extraction of silica from rice husk ash (RHA) for the encapsulation of aflatoxin B1 antibody (Ab-AFB1) and its application as a matrix in immunoaffinity columns (IACs) were achieved. The RHA extraction was performed using 4 M NaOH, which yielded sodium silicate (Na2SiO3) for the synthesis of silica gel. The obtained silica was used for encapsulating Ab-AFB1 using the sol-gel technique. One milliliter of 1 M Na2SiO3:H2O:H3PO4 (0.43:0.11: 0.46) could generate silica gel that was suitable for encapsulating 1.36 mg of Ab-AFB1 at pH 7. After 48 hours of aging, the silica gel modified with AbAFB1 (SG-Ab-AFB1) was ground, and packed as the matrix in the IAC for aflatoxins purification. The modified silica gel was characterized using FTIR and SEM. The properties of IAC with SG-Ab-AFB1 were investigated by evaluating AF recovery, binding capacity, and reusability. The recovery of AFB1 was 94.11 ± 4.62%. In addition to AFB1 recovery, the column also retained AFB2, AFG1, and AFG2 with recovery values of 98.22 ± 3.74%, 92.22 ± 7.62%, and 83.00 ± 6.31%, respectively. This column, which contained 0.5 g of SG-AbAFB1 had a binding capacity of approximately 50 ng of AFs per column, and could be reused at least 5 times with a recovery of more than 80%. |
| Group of Knowledge | : | Kimia |
| Original Language | : | English |
| Level | : | Internasional |
| Status | : |
Published
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| 1 |
J11_9_2017_Application of silica extracted from rice husk ash for_Full Dokumen_1.pdf
Document Type : [PAK] Full Dokumen
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J11_5_2017_Application of silica extracted from rice husk ash for_JSM_Similarity.pdf
Document Type : [PAK] Cek Similarity
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| 3 |
j1192017application-of-silica-extracted-from-rice-husk-ash-forfull-dokumen1pdf.pdf
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