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Title : Association between prognostic factors and the outcomes of patients infected with SARS-CoV-2 harboring multiple spike protein mutations
Author :

Prof. dr. Gunadi, Ph.D, Sp.BA., Subsp.DA(K) (1) dr. Mohamad Saifudin Hakim, M.Sc, Ph.D. (2) drh. Hendra Wibawa, M.Si., Ph.D. (3) MARCELLUS (4) dr. Ika Trisnawati, M.Sc., Sp.PD-KP (5) ENDAH SUPRIYATI (6) Afiahayati, S.Kom., M.Cs., Ph.D (7) dr. Riat El Khair, M.Sc., Sp.PK. (8) dr. Kristy Iskandar, M.Sc, Ph.D, Sp.A. Subsp.Neuro.(K) (9) dr. Siswanto, Sp.P(K) (10) Irene (11) dr. Nungki Anggorowati, Sp.PA(K)., Ph.D. (12) dr. Marselinus Edwin Widyanto Daniwijaya, Ph.D., Sp. MK (13) dr. Dwi Aris Agung Nugrahaningsih, M.Sc., Ph.D (14) YUNIKA PUSPA DEWI (15) Susan Simanjaya (16) RADEN AJENG DYAH AYU (17) HANA FAUZYYAH HANIFIN (18) ALVINA ALEXANDRA S (19) IRENE TANIA (20) CITA SHAFIRA AMALIA (21) I PUTU ADITIO ARTAYASA (22) HARIES RACHMAN (23) Herdiyanto Mulyawan (24) dr. Nur Rahmi Ananda, Sp.PD-KP. (25) dr. Eggi Arguni, M.Sc., Ph.D., Sp.A(K). (26) Prof. dr. Titik Nuryastuti, M.Si., Ph.D, SpMK(K) (27) Prof. dr. Tri Wibawa, Ph.D., Sp.MK(K) (28)
Date : 1 2021
Keyword : COVID-19 outcomes,comorbidity,genomic surveillance,multiple spike protein mutations,phylogenetic analysis,SARS-CoV-2 variants,whole-genome sequencing COVID-19 outcomes,comorbidity,genomic surveillance,multiple spike protein mutations,phylogenetic analysis,SARS-CoV-2 variants,whole-genome sequencing
Abstract : The outcome of SARS-CoV-2 infection is determined by multiple factors, including the viral, host genetics, age, and comorbidities. This study investigated the association between prognostic factors and disease outcomes of patients infected by SARS-CoV-2 with multiple S protein mutations. Fifty-one COVID-19 patients were recruited in this study. Whole-genome sequencing of 170 full-genomes of SARS-CoV-2 was conducted with the Illumina MiSeq sequencer. Most patients (47%) had mild symptoms of COVID-19 followed by moderate (19.6%), no symptoms (13.7%), severe (4%), and critical (2%). Mortality was found in 13.7% of the COVID-19 patients. There was a significant difference between the age of hospitalized patients (53.4 ± 18 years) and the age of non-hospitalized patients (34.6 ± 19) (p=0.001). The patients’ hospitalization was strongly associated with hypertension, diabetes, and anticoagulant and were strongly significant with the OR of 17 (95% CI=2-144; p=0.001), 4.47 (95% CI=1.07-18.58; p=0.039), and 27.97 (95% CI=1.54-507.13; p=0.02), respectively; while the patients’ mortality was significantly correlated with patients’ age, anticoagulant, steroid, and diabetes, with OR of 8.44 (95% CI=1.5-47.49; p=0.016), 46.8 (95% CI=4.63-472.77; p=0.001), 15.75 (95% CI=2-123.86; p=0.009), and 8.5 (95% CI=1.43-50.66; p=0.019), respectively. This study found the clade: L (2%), GH (84.3%), GR (11.7%), and O (2%). Besides the D614G mutation, we found L5F (18.8%), V213A (18.8%), and S689R (8.3%). No significant association between multiple S protein mutations and the patients’ hospitalization or mortality. Multivariate analysis revealed that hypertension and anticoagulant were the significant factors influencing the hospitalization and mortality of patients with COVID-19 with an OR of 17.06 (95% CI=2.02-144.36; p=0.009) and 46.8 (95% CI=4.63-472.77; p=0.001), respectively. Moreover, the multiple S protein mutations almost reached a strong association with patients’ hospitalization (p=0.07). We concluded that hypertension and anticoagulant therapy have a significant impact on COVID-19 outcomes. This study also suggests that multiple S protein mutations may impact the COVID-19 outcomes. This further emphasized the significance of monitoring SARS-CoV-2 variants through genomic surveillance, particularly those that may impact the COVID-19 outcomes.
Group of Knowledge : Ilmu Kedokteran Umum
Original Language : English
Level : Internasional
Status :
Published
Document
No Title Document Type Action
1 Form-L1-9th-ScientificReports_SARSCoV2.pdf
Document Type : Dokumen Pendukung Karya Ilmiah (Hibah, Publikasi, Penelitian, Pengabdian)
Dokumen Pendukung Karya Ilmiah (Hibah, Publikasi, Penelitian, Pengabdian) View
2 Fulldoc_association between prognostic and outcome sars cov2.pdf
Document Type : [PAK] Full Dokumen
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3 Association between prognostic factors and the outcomes of patients infected with SARS?CoV?2 harboring multiple spike protein mutations.pdf
Document Type : [PAK] Cek Similarity
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4 8_BuktiKorespondensi_MultipleSpikeMutationsSARSCoV2_rev.pdf
Document Type : [PAK] Bukti Korespondensi Penulis
[PAK] Bukti Korespondensi Penulis View
5 s41598-021-00459-4.pdf
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