ACADSTAFF UGM

CREATION
Title : Clinical Assessment of Intravenous Endothelial Progenitor Cell Transplantation in Dogs.
Author :

Seok Hee Lee (1) Jeong Chan Ra (2) Hyun Ju Oh (3) Min Jung Kim (4) drh. Erif Maha Nugraha Setyawan, M.Sc., Ph.D. (5) Yoo Bin Choi (6) Jung Won Yang (7) Sung Keun Kang (8) Seung Hyup Han (9) Geon A. Kim (10) Byeong Chun Lee (11)
Date : 0 2019
Keyword : dogs, physiological assessment, endothelial progenitor cells, stem cells, transplantation dogs, physiological assessment, endothelial progenitor cells, stem cells, transplantation
Abstract : Endothelial progenitor cells (EPCs) have been applied for cell therapy because of their roles in angiogenesis and neovascularization in ischemic tissue. However, adverse responses caused by EPC therapy have not been fully investigated. In this study, a human peripheral blood sample was collected from a healthy donor and peripheral blood mononuclear cells were separated using Ficoll-Hypaque. There were four experimental groups: 10 ml saline infusion group (injection rate; 3 ml/min), 10 ml saline bolus group (injection rate; 60 ml/min), 10 ml EPCs infusion group (2 x 105 cells/ml, injection rate; 3 ml/min), 10 ml EPCs bolus group (2 105 cells/ml, injection rate; 60 ml/min). Clinical assessment included physical examination and laboratory examination for intravenous human EPC transplantation in dogs. The results revealed no remarkable findings in vital signs among the dogs used. In blood analysis, platelet counts in saline infusion groups were significantly higher than in the EPC groups within normal ranges, and no significant differences were observed except Kþ, Cl– and blood urea nitrogen/urea. In ELISA assay, no significant difference was observed in serum tumor necrosis factor alpha. The serum concentration of vascular endothelial growth factor was significantly higher in EPC groups than in saline groups, and interleukin 10 was significantly up-regulated in the EPC infusion group compared with other groups. In conclusion, we demonstrated that no clinical abnormalities were detected after intravenous transplantation of human EPCs in dogs. The transplanted xenogenic EPCs might be involved in antiinflammatory and angiogenic functions in dogs.
Group of Knowledge : Kedokteran Hewan
Original Language : English
Level : Internasional
Status :
Published
Document
No Title Document Type Action
1 1 COVER JURNAL_Clinical Assessment of Intravenous.pdf
Document Type : [PAK] Halaman Cover
[PAK] Halaman Cover View
2 2 EDITORIAL BOARD-Clinical Assessment of Intravenous.pdf
Document Type : [PAK] Halaman Editorial
[PAK] Halaman Editorial View
3 3 TABLE OF CONTENT_Clinical Assessment of Intravenous.pdf
Document Type : [PAK] Daftar Isi
[PAK] Daftar Isi View
4 4 ARTIKEL JOURNAL_Clinical Assessment of Intravenous.pdf
Document Type : Dokumen Pendukung Karya Ilmiah (Hibah, Publikasi, Penelitian, Pengabdian)
Dokumen Pendukung Karya Ilmiah (Hibah, Publikasi, Penelitian, Pengabdian) View
5 5 SJR 1_042 Q1(2019) .pdf
Document Type : Dokumen Pendukung Karya Ilmiah (Hibah, Publikasi, Penelitian, Pengabdian)
Dokumen Pendukung Karya Ilmiah (Hibah, Publikasi, Penelitian, Pengabdian) View
6 FULL DOKUMEN Clinical Assessment of Intravenous Endothelial Progenitor Cell Transplantation in Dogs.pdf
Document Type : [PAK] Full Dokumen
[PAK] Full Dokumen View
7 SIMILARITAS_Clinical Assessment of Intravenous.pdf
Document Type : [PAK] Cek Similarity
[PAK] Cek Similarity View