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CREATION
Title : Detection of Trypanosoma evansi in a naturally infected cat in Indonesia using bioassay and molecular techniques
Author :

Dr. drh. Dwi Priyowidodo, M.P. (1) Dr. drh. Ana Sahara, M.Si. (2) Dr. drh. Joko Prastowo, M.Si. (3) Prof. Dr. drh. Raden Wisnu Nurcahyo (4) Lintang Winantya Firdausy (5)
Date : 20 2023
Keyword : bioassay,feline,internal transcribed spacer-1, molecular detection,Trypanosoma evansi bioassay,feline,internal transcribed spacer-1, molecular detection,Trypanosoma evansi
Abstract : Background and Aim: Surra in domestic cats is caused by Trypanosoma brucei and Trypanosoma evansi. However, molecular diagnostic approaches are required owing to similarities in their morphology. In Yogyakarta, a domestic cat was diagnosed with trypanosomiasis; however, the causative species was undetermined. Therefore, we aimed to molecularly and biologically identify the isolate. Materials and Methods: Ten mice were inoculated intraperitoneally with trypanosomatid to determine their biological characteristics, and the parasitemia pattern and viability of the animals were observed. Internal transcribed spacer (ITS)-1 amplification was used to assess the molecular characteristics of trypanosomatid. Results: The prepatent period of this trypanosomatid is between 2 and 4 dpi, whereas the life span of mice is approximately 4–10 dpi. Morphologically, the trypomastigote in the cat blood smear had long slender and intermediate shapes. However, only the long slender form was detected. Among the total of 410 nucleotides (NT) of ITS-1 sequences, 25 NT substitutions differed between the cat and mouse isolates. Phylogenetic analysis revealed that both samples had a close genetic relationship with T. evansi.
Group of Knowledge : Kedokteran Hewan
Original Language : English
Level : Internasional
Status :
Published
Document
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1 21.pdf
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2 Plagiarism_check.pdf
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3 Dwi Priyowidodo - Acceptace (1).pdf
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4 Korespondensi Trypano Kucing_Submit.pdf
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5 Korespondensi Trypano Kucing_Revision_compressed.pdf
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6 Korespondensi Trypano Kucing-ok_compressed.pdf
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7 Sertifikat Publikasi.pdf
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8 Full Dokumen.pdf
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9 Detection of Trypanosoma evansi in a naturally infected cat in Indonesia using bioassay and molecular techniques.pdf
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10 BUKTI KORESPONDENSI DP.pdf
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11 L1_DP.pdf
Document Type : Dokumen Pendukung Karya Ilmiah (Hibah, Publikasi, Penelitian, Pengabdian)
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