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Title : GENOTYPIC AND PHENOTYPIC CHARACTERIZATION OF Alcaligenes javaensis JG3 POTENTIAL AS AN EFFECTIVE BIODEGRADER
Author :

Stalis Norma Ethica (1) Oedjijono Oedjijono (2) Prof. Dr. Endang Semiarti, M.S., M.Sc. (3) Ir. Jaka Widada, M.P., Ph.D. (4) Prof. Tri Joko Raharjo, S.Si., M.Si., Ph.D. (5)
Date : 0 2018
Keyword : Alcaligenes, biodegradation, degenerate PCR, glpK, glycerol metabolism Alcaligenes, biodegradation, degenerate PCR, glpK, glycerol metabolism
Abstract : Utilization of glycerol by lipase producing bacteria offers great benefits for fat and oil waste degradation and waterwaste treatment. Nevertheless, there have been lack of reports about the availability of non-pathogenic, lipase producing bacteria, which could naturally degrade glycerol produced from the lipolysis process by lipase. This study reported a newly identified species of rhizobacteria, Alcaligenes javaensis JG3, which is not only able to produce high level of lipase, but also able to degrade glycerol molecules. Identification of strain JG3 was carried out using SEM (Scanning Electron Microscope), BD Phoenix 100 Automated Microbiology System and 16S rRNA gene analysis to determine its taxonomy status. The ability of the strain to metabolize glycerol was investigated both genotypically and phenotypically using degenerate PCR and a glycerol minimal medium. Identification test results showed that strain JG3 belongs to genus Alcaligenes, with the closest relationship with A. faecalis and A. aquatilis (96% nucleotide similarity maximum). Degenerate PCR resulted in a 248-bp sequence showing 93% similarity with glpK of Candidatus Sodalis pierantonius SOPE, a key gene involved in glycerol metabolism. In vitro glycerol utilization test result showed that Alcaligenessp. JG3 was able to grow on glycerol aerobically, but not anaerobically. It is concluded that Alcaligenessp. JG3 possesses genes coding for glycerol metabolism and this trait is phenotypically expressed, thus making the strain potential to be used as an effective fat and oil biodegrader. Keywords: Alcaligenes, biodegradation, degenerate PCR,
Group of Knowledge : Biologi (dan Bioteknologi Umum)
Original Language : English
Level : Internasional
Status :
Published
Document
No Title Document Type Action
1 GENOTYPIC AND PHENOTYPIC CHARACTERIZATION OF Alcaligenes javaensis JG3 POTENTIAL AS AN EFFECTIVE BIODEGRADER.pdf
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2 GENOTYPIC AND PHENOTYPIC CHARACTERIZATION OF Alcaligenes javaensis JG3 POTENTIAL AS AN EFFECTIVE BIODEGRADER.pdf
Document Type : [PAK] Cek Similarity
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3 hanjuang,+583+Stalis+Norma+(Genotypic).pdf
Document Type : [PAK] Full Dokumen
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