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Title : Growth Rate and Histamine Production of Citrobacter freundii CK01 in Various Incubation Temperatures
Author :

GRACE MARGARETA (1) Susana Endah Ratnawati, S.Pi., M.Si. (2) Indun Dewi Puspita, S.P., M.Sc. Ph.D. (3)

Date : 0 2020
Keyword : Citrobacter freundii, histamine,temperature,growth model Citrobacter freundii, histamine,temperature,growth model
Abstract : Improper handling and temperature fluctuations during postharvest of fish commonly allow the growth of histamine-forming bacteria (HFB) that may cause histamine poisoning. Citrobacter freundii CK01 is one of the HFB isolated from Skipjack landed on Sadeng, Yogyakarta. This study aimed to determine the temperature effect on the growth rate and histamine production by C. freundii CK01. Bacterial growth and histamine production was tested in tuna fish infusion broth (TFIB) at 5, 15, 30 and 40°C. The bacterial growth was tested using Total Plate Count method, while histamine was determined using Thin Layer Chromatography and densitometry method. The primary model for bacterial growth was plotted with incubation time using DMFit referred to Baranyi & Roberts model. The secondary model was converted from growth rate and modeled using Ratkowsky Square Root Model. The equation for growth rate of C. freundii CK01 was ?max=[0.0105 (T + 13.886)]2 (Root Mean Square Error <10%). Histamine production reached the highest concentration at 15°C in 96 hours up to 117,13 ppm. This study shows that temperature affected the growth rate and histamine production of C. freundii, indicating the importance of maintaining the low temperature stability during handling of skipjack.
Group of Knowledge :
Level : Internasional
Status :
Published
Document
No Title Document Type Action
1 Prosiding ISMFR2019_Grace.pdf
Document Type : Bukti Published
Bukti Published View
2 Margareta, et al 2020_ Growth Rate.pdf
Document Type : [PAK] Full Dokumen
[PAK] Full Dokumen View
3 Growth Rate and Histamine Production of Klebsiella sp_ CK02 Isolated from Skipjack Tuna Compared with Morganella morganii ATCC 25830 at Various Incubation Temperatures.pdf
Document Type : Cek Similarity
Cek Similarity View
4 Sertifikat ISMFR 2019_Grace.pdf
Document Type : Artikel dan Sertifikat/Bukti Kehadiran/Pasport (jika tidak ada sertifikat)
Artikel dan Sertifikat/Bukti Kehadiran/Pasport (jika tidak ada sertifikat) View
5 Publication acceptance-Grace.pdf
Document Type : Artikel dan Sertifikat/Bukti Kehadiran/Pasport (jika tidak ada sertifikat)
Artikel dan Sertifikat/Bukti Kehadiran/Pasport (jika tidak ada sertifikat) View
6 Full dokumen_Prosiding ISMFR 2019_Grace.pdf
Document Type : [PAK] Full Dokumen
[PAK] Full Dokumen View