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Title : In vitro bioactivity of 3D microstructure hydroxyapatite/collagen based-egg white as an antibacterial agent
Author :

DIANA JULAIDY PATTY (1) Dr.Sc. Ari Dwi Nugraheni, S.Si., M.Si. (2) Prof. drg. Ika Dewi Ana, M.Kes., Ph.D. (3) Prof. Dr. Eng. Yusril Yusuf, S.Si., M.Si., M.Eng. (4)

Date : 18 2022
Keyword : antibacterial activity, collagen, egg-white, hydroxyapatite, in vitro antibacterial activity, collagen, egg-white, hydroxyapatite, in vitro
Abstract : The present study aims to design 3D scaffold hydroxyapatite (HA)/collagen (Coll) based egg-white (EW) as antibacterial properties. The calcium source in HA synthesis derived from the Pinctada maxima shell cultivated on Bali Island has proven biocompatibility, and the compressive strength exceeded human bone. HA synthesis by precipitation with heat treatment in oven-dried at 80°C (HA-80) and annealed at 900°C (HA-900), has crystallinity 48% and 85%, respectively, were used for scaffold design. The physicochemical properties of X-ray diffractometer spectra showed that increasing temperature affected the crystallinity and HA phase formed. Furthermore, the crystal structure of HA changed in nanocomposite due to the substitution of Coll and EW, and the Fourier transform infrared spectroscopy spectra confirmed that the absorption peak of the phosphate group (1027–1029 cm−1) decreased intensity, presumably by protein binding of EW and Coll. The cell viability of HA/Coll/EW in 24, 48, and 72 h incubation period was 112.34 ± 4.36, 104.89 ± 3.41, 72.88 ± 6.85, respectively. The decreases of cell viability due to high cell density and reduced nutrients in wells. Antibacterial activity of HA/Col/EW exhibited a strong zone of inhibition against bacteria causing periodontitis; Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum, and Staphylococcus aureus.
Group of Knowledge : Bidang IPA Lain Yang Belum Tercantum
Original Language : English
Level : Internasional
Status :
Published
Document
No Title Document Type Action
1 49_ J Biomed Mater Res - 2022 - Patty - In vitro bioactivity of 3D microstructure hydroxyapatite collagen based?egg white as an_compressed.pdf
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2 L1_Diana and Yusril JBMR.pdf
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3 53 Similaritas In vitro bioactivity of 3D microstructure hydroxyapatite collagen based?egg white as an.pdf
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4 Diana_JBMR_2022.pdf
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5 In vitro bioactivity of 3D microstructure hydroxyapatite_collagen based-egg white as an antibacterial agent (1).pdf
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