| Abstract |
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Lead uptake capacity of four lead-tolerant thermophilic bacteria Aeribacillus pallidus strains MRP112, MRP148, MRP272, and MRP278, originally isolated from Merapi volcano, were examined for their potential as an absorbent to uptake lead (Pb) from an aqueous solution. The growth profile and Pb uptake capacity were evaluated in Luria Bertani (LB) medium supplemented with Pb 100 mg/L. The optimum condition was evaluated under various analytical parameters, including temperature (55°C, 60°C, 65°C), pH (5, 6, 7), and contact time (6, 15, 24 h). The interaction of bacterial cell surface with ion Pb was proven using Fourier transform infrared spectroscopy (FT-IR). The optimal growth of all strains was observed at 55°C and pH 7. Aeribacillus pallidus MRP112 showed the highest uptake capacity of 97.41 ± 0.82 mg/g at 55°C, pH 5 for 15 h. FT-IR analysis displayed involved various functional groups such as hydroxyl, carboxyl, amine, and amide group ligands of protein in binding Pb ion. The A. pallidus strain represents a good candidate for the bioremediation of Pb-contaminated environment. |
