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Title : Optimization of Colloidal Chitin and Inoculum Concentration in Chitinase Production by Streptomyces sp. PB2 Using Response Surface Methodology
Author :

ERI PRAMESTI (1) Indun Dewi Puspita, S.P., M.Sc. Ph.D. (2)

Date : 0 2020
Keyword : inoculum colloidal chitin, RSM, Streptomyces sp. PB2,chitinase inoculum colloidal chitin, RSM, Streptomyces sp. PB2,chitinase
Abstract : Chitinase is a glycosyl hydrolase enzyme capable of catalyzing the hydrolysis of chitin into oligomers and monomers. Bacteria is one of the sources for chitinase and the modification process of production to enhance enzymatic reaction are continously developed. Around 90-99% of the chitinolytic bacteria population are Actinomycetes. Streptomyces sp. is one of Actinomycetes group that shows high activity in hydrolyzing chitin. This study aimed to find the optimal conditions of Streptomyces sp. PB2 for producing chitinase at various colloidal chitin (0,5%, 1%, 1,5%) and inoculum (0.5%, 1%, 1,5%) concentration in the chitin broth medium using Respons Surface Methodology (RSM). The examined parameters included Total Plate Count (CFU/ml) and chitinase activity (U/ml). Chitinase activity was statistically analyzed by MiniTab 17 to obtain a mathematical model, then were validated. The mathematical model of chitinase activity was Y = - 0.00075 + 0.00056 K + 0.00067 I, with the optimum colloidal chitin concentration (K) of 1.5% and inoculum concentration (I) of 1.5%. This study shows that colloidal chitin and inoculum concentration are important factors to be optimized in order to reach an optimal production of bacterial chitinase.
Group of Knowledge :
Level : Internasional
Status :
Published
Document
No Title Document Type Action
1 Indun Dewi Puspita - Eri.pdf
Document Type : Bukti Accepted
Bukti Accepted View
2 PA-18-190721073050_NF_Revisi Akhir.pdf
Document Type : Bukti Draft
Bukti Draft View
3 Prosiding ISMFR2019_Eri.pdf
Document Type : Bukti Published
Bukti Published View
4 prosiding full_Eri Pramesti.pdf
Document Type : [PAK] Full Dokumen
[PAK] Full Dokumen View
5 prosiding full_Eri Pramesti.pdf
Document Type : Cek Similarity
Cek Similarity View
6 Sertifikat ISMFR 2019_Eri.pdf
Document Type : Artikel dan Sertifikat/Bukti Kehadiran/Pasport (jika tidak ada sertifikat)
Artikel dan Sertifikat/Bukti Kehadiran/Pasport (jika tidak ada sertifikat) View
7 Full dokumen_Prosiding ISMFR 2019_Eri.pdf
Document Type : [PAK] Full Dokumen
[PAK] Full Dokumen View