ACADSTAFF UGM
| Title | : | Preliminary Study on Health-Associated Effects of Human-origin Lactic Acid Bacteria as Probiotics |
| Author | : |
Prof. Widodo, S.P., M.Sc. Ph.D. (1) Prof. Dra. Tutik Dwi Wahyuningsih, M.Si., Ph.D. (2) |
| Date | : | 2014 |
| Keyword | : | Human-origin probiotics, Indonesian infant, lactic acid bacteria Human-origin probiotics, Indonesian infant, lactic acid bacteria |
| Abstract | : | The aim of this study was to isolate, identify and to evaluate the human-origin lactic acid bacteria from feces of breast-fed Indonesian infant as probiotic. Of the seventeen isolates obtained, five had been identified with three isolates AA, BE and BK were strains of Pediococcus acidilactici, and two isolates AP and AG were strains of Lactobacillus casei. Probiotic assays showed more than 80% of cells of Pediococcus acidilactici strain AA, BE and BK were viable after grown on pH 2.0 for 90 min, and around 80% of cells were survived on media supplemented with bile salt 1.5% for 2 h. All of three strains had high adhesion capacity as seen by more than 75% of cells attached on pig gastric mucin. Investigation of strains AA, BE and BK to grow on inulin showed only strain BE was able to consume inulin as the only carbon source. Meanwhile, study on probiotic capability of Lactobacillus casei strain AP and AG showed 80% of cells survived at pH 2.0 for 90 min, while survival of strains after growing at media supplemented with 1.5% bile salts was more than 70%. These two strains showed high level (70%) attachment on gastric mucin.Both strains AP and AG were also able to grow normally using inulin as the only carbon source. Study in vitro showed all of five strains had ability to inhibit the growth of Shigella flexnerii, Eschericia coli, Staphylococcus aureus and Enterococcus faecalis. Of these five strains, only strain AP was able to synthesize conjugated linoleic acid (CLA) from linoleic acid. Study in vivo in rats (Rattus norvegicus) of three selected strain AP, AG and BE increased total lactic acid bacteria (LAB) in feces (P<0.05) and decreased level of total cholesterol in blood serum (P<0.05). Feed supplementation with Lactobacillus strain AP increased 4.36±2.47% of total LAB followed by strain AG at 9.14±1.28% and strain BE at 8.13±4.43%. Meanwhile feed supplementation with strain AP, AG and BE showed a cholesterol reduction in blood serum at 22.85±5.34, 40.89±3.63 and 23.18±4.19%, respectively. Based on this study, Lactobacillus casei strain AP was chosen for further study on other health-associated effects and human clinical study. |
| Group of Knowledge | : | Nutrisi dan Makanan Ternak |
| Level | : | Internasional |
| Status | : |
Published
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| No | Title | Action |
|---|---|---|
| 1 |
2014 ICoBM Widodo et al.pdf
Document Type :
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