ACADSTAFF UGM
| Title | : | Sensitivity and Specificity of an All-in-One Cartridge-Based Dengue Real-Time RT-PCR for Point-of-Care Detection and Serotyping of Dengue Virus in Samples From Indonesian Patients |
| Author | : |
Marsha S. Santoso (1) Prof. dr. Eggi Arguni, M.Sc., Ph.D., Sp.A(K). (2) Bunga Rana (3) Mercy E. Adiniko (4) Dionisius Denis (5) Endah Supriyati (6) dr. Citra Indriani, MPH. (7) Leily Trianty (8) dr. Riris Andono Ahmad, MPH, Ph.D (9) Rintis Noviyanti (10) R. Tedjo Sasmono (11) |
| Date | : | 0 2025 |
| Keyword | : | Dengue, Diagnostic accuracy, Point-of-Care testing, Sensitivity, Specificity Dengue, Diagnostic accuracy, Point-of-Care testing, Sensitivity, Specificity |
| Abstract | : | Background: Dengue is a systemic, viral, mosquito-borne infection that continues to be a major public health issue in endemic regions in tropical and subtropical climates. Accurate tests for rapid diagnosis in point-of-care settings are important to reduce the fatality rates of severe dengue. We evaluated the diagnostic accuracy of the Standard M10 DENV 1-4 system (SD Biosensor, Gyeonggi, Korea), which is a cartridge-based, automated system that integrates nucleic acid extraction, reverse transcription-PCR (RT-PCR) amplification, and detection of dengue virus (DENV) serotypes. Methods: This was a retrospective diagnostic evaluation study. The index test, Standard M10 DENV 1-4, was evaluated using 320 dengue-positive and 279 dengue-negative archived samples. The reference tests were a combination of Centers for Disease Control and Prevention (CDC) DENV 1-4 real-time RT-PCR, dengue NS1 antigen and IgM antibody detection, and DENV whole-genome sequencing. Results: The overall sensitivity and specificity of Standard M10 DENV 1-4 were 94% and 100%, respectively. By serotype, the highest sensitivity was 100% for DENV-1, and the lowest was 82% for DENV-4. The overall between the CDC RT-PCR dengue serotyping method and the Standard M10 DENV 1-4 was 95%. Standard M10 DENV 1-4 RT-PCR had comparable sensitivity and specificity to CDC DENV RT-PCR. Conclusions: Based on its commensurate performance to an established RT-PCR method combined with additional benefits of convenient storage and transport, easy use, and rapid processing, the Standard M10 DENV 1-4 system has potential for DENV detection and serotyping in point-of-care settings. |
| Group of Knowledge | : | Ilmu Kedokteran Tropis |
| Original Language | : | Arabic |
| Level | : | Internasional |
| Status | : |
Published
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ALM3614.pdf
Document Type : [PAK] Full Dokumen
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