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Title : The preparation and characterization of Alginate–Chitosan membranes as solid support for BTB and urease entrapment
Author :

Dhony Hermanto (1) Prof. Drs. Mudasir, M.Eng., Ph.D. (2) Drs. Dwi Siswanta, M.Eng., Ph.D. (3) Bambang Kuswandi (4) Nurul Ismillayli (5)

Date : 0 2020
Keyword : Alginate,Application,Characterization,Chitosan,Membrane,Preparation Alginate,Application,Characterization,Chitosan,Membrane,Preparation
Abstract : A study of alginate-chitosan membrane synthesize was done. The membrane was prepared by mixing alginate and chitosan hydrosols (1:1 in mass ratio) at pH approximately 5.28. Then it was applied for matrix immobilization of urease and bromothymol blue (BTB) by entrapment technique. The physical, chemical, thermal properties of alginate– chitosan membrane and their impact on immobilized urease activity were investigated. The polymer products were characterized by Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction (XRD), Thermogravimetric Analysis (TGA), Differential Thermal Analysis (DTA), and Scanning Electron Microscopy (SEM). It’s showed that alginate-chitosan membrane was formed by electrostatic interaction. The obtained membran has better mechanical properties than original alginat and chitosan membranes. The immobilization urease into alginate-chitosan membrane retained the catalytic activity of the enzyme, as confirmed by color change of BTB indicator after membrane was immersed in substrate solution (urea). Therefore, alginate-chitosan membrane has good characteristics as matrix of enzyme immobilization.
Group of Knowledge : Kimia
Original Language : English
Level : Internasional
Status :
Published
Document
No Title Document Type Action
1 527-1748-1-PB-1_Donny_Molekul 2020.pdf
Document Type : Bukti Published
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2 The Preparation and Characterization of Alginate–Chitosan Membranes as Solid Support for BTB and Urease Entrapment (2).pdf
Document Type : [PAK] Cek Similarity
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3 527-1748-1-PB.pdf
Document Type : [PAK] Full Dokumen
[PAK] Full Dokumen View