ACADSTAFF UGM
| Title | : | The use of Fe3O4 magnetic nanoparticles as the active layer to detect plant's DNA with surface plasmon resonance (SPR) based biosensor |
| Author | : |
Devy Pramudyah Wardani, S.Si., M.Sc. (2) Dr.Eng. Edi Suharyadi, S.Si., M.Eng. (3) Prof. Dr. Kamsul Abraha (4) |
| Date | : | 2016 |
| Keyword | : | SPR SPR |
| Abstract | : | The surface plasmon resonance (SPR) phenomenon has been observed on the silver-coated prism and Fe3O4 magnetic nanoparticles for detecting plant’s DNA. Fe3O4 nanoparticles were synthesized using temperature of 30°C and they were coated with PEG4000. The composite of Fe3O4+PEG4000 has a function as a DNA binding active layer and for optimizing the detection ability of SPR. SPR-based biosensor in this work using Kretschmann configuration and He- Ne laser beam (? = 632.8 nm). The plant’s DNA used in this study has ratio 1.828. The plant’s DNA could be detected by SPR-based biosensor with the ratio of composite Fe3O4 : PEG4000 is 1:1. This work was done for the earlier study to approve that SPR can be used to detect DNA with the active layer on it (in this case: composite Fe3O4+PEG4000). Thus, the shift of SPR-angle was influenced by the DNA ratio, concentration of Fe3O4 and its refractive index. |
| Group of Knowledge | : | |
| Level | : | Internasional |
| Status | : |
Published
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| 1 |
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